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跟着NatureMetabolism学作图：R语言ggplot2转录组差异表达火山图

语言 差异 转录 表达 ggplot2 跟着 作图 火山
2023-06-13 09:16:32 时间

论文

Independent phenotypic plasticity axes define distinct obesity sub-types

https://www.nature.com/articles/s42255-022-00629-2#Sec15

s42255-022-00629-2.pdf

论文中没有公开代码，但是所有作图数据都公开了，我们可以试着用论文中提供的数据模仿论文中的图

今天的推文重复一下论文中的Fig3b 差异表达火山图，之前也有推文介绍过火山图，今天的推文主要学习的一个知识点是利用latex2exp这个R包添加文本，包括

  • 上下标
  • 换行 换行的基本写法
ggplot()+
  geom_point(aes(x=1,y=1))+
  labs(x=TeX(r"(\overset${ABCDEF}{abcde}$)"))

image.png

火山图的部分示例数据

image.png

读取数据 

library(readr)
df<-read_tsv("data/20220921/fig3b.txt")
head(df)
colnames(df)

添加差异表达的分组 

df %>% 
  mutate(change=case_when(
    log2FoldChange > 1 & pvalue < 0.05 ~ "Up",
    log2FoldChange < -1 & pvalue < 0.05 ~ "Down",
    TRUE ~ "Not Sig"
  )) -> new.df

table(new.df$change)
new.df %>% 
  filter(-log10(pvalue)>8) -> new.text.label

这里没有找到论文中差异表达的标准，这里是我随便写的

作图代码 

library(ggplot2)
library(ggrepel)
library(latex2exp)
ggplot(data=new.df,aes(x=log2FoldChange,y=-log10(pvalue)))+
  geom_point(aes(color=change))+
  scale_color_manual(values = c("Down"="#3a53a4",
                                "Not Sig"="#aaaaaa",
                                "Up"="#7acde4"),
                     labels=c("Down"=TeX(r"(\textit{Nnat}${^+}$${^/}$${^-}$${^p}$-\textit{Heavy} depleted)"),
                              "Not Sig"="Not Significant",
                              "Up" = TeX(r"(\textit{Nnat}${^+}$${^/}$${^-}$${^p}$-\textit{Heavy} enriched)")))+
  theme_classic()+
  theme(legend.position = c(0.2,0.9),
        legend.text.align = 0,
        legend.title = element_blank())+
  geom_text_repel(data=new.text.label,
                  aes(x=log2FoldChange,y=-log10(pvalue),
                      label=mgi_symbol))+
  labs(x=TeX(r"(\overset${\textit{Nnat}{^+}{^/}{^-}{^p}-\textit{Lean}$ versus $\textit{Nnat}{^+}{^/}{^-}{^p}-\textit{Giant}}{(log{_2}$ fold $change)}$)"),
       y=TeX(r"(-log${_1}{_0}$ {(}\textit{P}{ value}{)})"))

image.png

制作封面图 

p1<-ggplot(data=new.df,aes(x=log2FoldChange,y=-log10(pvalue)))+
  geom_point(aes(color=change))+
  scale_color_manual(values = c("Down"="#3a53a4",
                                "Not Sig"="#aaaaaa",
                                "Up"="#7acde4"),
                     labels=c("Down"=TeX(r"(\textit{Nnat}${^+}$${^/}$${^-}$${^p}$-\textit{Heavy} depleted)"),
                              "Not Sig"="Not Significant",
                              "Up" = TeX(r"(\textit{Nnat}${^+}$${^/}$${^-}$${^p}$-\textit{Heavy} enriched)")))+
  theme_classic()+
  theme(legend.position = c(0.2,0.9),
        legend.text.align = 0,
        legend.title = element_blank())+
  geom_text_repel(data=new.text.label,
                  aes(x=log2FoldChange,y=-log10(pvalue),
                      label=mgi_symbol))+
  labs(x=TeX(r"(\overset${\textit{Nnat}{^+}{^/}{^-}{^p}-\textit{Lean}$ versus $\textit{Nnat}{^+}{^/}{^-}{^p}-\textit{Giant}}{(log{_2}$ fold $change)}$)"),
       y=TeX(r"(-log${_1}{_0}$ {(}\textit{P}{ value}{)})"))

p2<-ggplot(data=new.df,aes(x=log2FoldChange,y=-log10(pvalue)))+
  geom_point(aes(color=change))+
  scale_color_manual(values = c("Down"="red",
                                "Not Sig"="#aaaaaa",
                                "Up"="darkgreen"),
                     labels=c("Down"=TeX(r"(\textit{Nnat}${^+}$${^/}$${^-}$${^p}$-\textit{Heavy} depleted)"),
                              "Not Sig"="Not Significant",
                              "Up" = TeX(r"(\textit{Nnat}${^+}$${^/}$${^-}$${^p}$-\textit{Heavy} enriched)")))+
  theme_classic()+
  theme(legend.position = c(0.2,0.9),
        legend.text.align = 0,
        legend.title = element_blank())+
  geom_text_repel(data=new.text.label,
                  aes(x=log2FoldChange,y=-log10(pvalue),
                      label=mgi_symbol))+
  labs(x=TeX(r"(\overset${\textit{Nnat}{^+}{^/}{^-}{^p}-\textit{Lean}$ versus $\textit{Nnat}{^+}{^/}{^-}{^p}-\textit{Giant}}{(log{_2}$ fold $change)}$)"),
       y=TeX(r"(-log${_1}{_0}$ {(}\textit{P}{ value}{)})"))

library(patchwork)
pdf(file = "Rplot03.pdf",width = 14.1,height = 6)
p1+p2
dev.off()

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